Recombinant Human epidermal growth factor Protein, Tag Free, Animal Free

• Cat.No.: EGF-06H
• Product Overview: Recombinant human EGF (971-1023aa) protein without tag was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Produced using nonanimal reagents in an animal-free laboratory.

Specification

• Description: This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
• Source: E. coli
• Species: Human
• Tag: Non
• Protein length: 971-1023aa
• Form: Liquid
• Bio-activity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 range ≤ 0.1 ng/mL.
• Molecular Mass: 6.3 kDa
• AA Sequence: MNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRGeneralReferences
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4)
• Concentration: 1 mg/mL (determined by Bradford assay)
• References: 1. Riese., et al (1998 ) Bioessays. 20: 41-48.
  2. Cohen S., (1983) Cancer. 15: 1787-1791.
  3. Carpenter G., et al (1979) Annu. Rev. Biochem., 48: 193-216.

Gene Information

• Gene Name: EGF epidermal growth factor [ Homo sapiens (human) ]
• Official Symbol: EGF
• Synonyms: EGF; epidermal growth factor; URG; HOMG4; pro-epidermal growth factor; beta-urogastrone
• Gene ID: 1950
• mRNA Refseq: NM_001963
• Protein Refseq: NP_001954
• MIM: 131530
• UniProt ID: P01133