Recombinant Phleum pratense Phl p 5a Allergen, His tagged, Biotinylated

• Cat.No.: Phl p 5a-044P
• Product Overview: The Phleum pratense major allergen Phl p 5 has been prepared as the recombinant mature allergen fused to a his-tag in its N-terminus and monobiotinylated in vivo.

Specification

• Species: Phleum pratense
• Source: E.coli
• Tag: His
• Description: Phl p 5a is a major allergen of grass pollen (Phleum pratense). It has been prepared as the recombinant mature allergen fused to a his-tag.
• Tag: N-His
• Conjugation/Label: Biotin
• Molecular Mass: Determined by SDS-PAGE, the protein band is at the molecular marker of 45 kDa and the dimer is between the molecular markers of 66 and 116 kDa, while relative molecular mass calculated from amino acid sequence is 40,257.84 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, CE, NP, PO
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM phosphate buffer pH 6, 0.15 M NaCl and 0.1% polyoxyethylene (10) tridecyl ether
• Concentration: 1.04 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Julia Hecker et al. Generation and epitope analysis of human monoclonal antibody isotypes with specificity for the timothy grass major allergen Phl p 5a. 2011, Molecular Immunology 48:1236-1244.
  2. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.