Recombinant Pan-species (General) LT Protein, His-tagged

• Cat.No.: LT-2764P
• Product Overview: Recombinant Pan-species (General) LT Protein (Lys131-Glu260) with a N-His tag was expressed in E. coli.

Specification

• Species: Pan-species
• Source: E.coli
• Tag: His
• ProteinLength: Lys131-Glu260
• Description: The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition, the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 18.8 kDa
  Accurate Molecular Mass: 19 kDa
• Purity: > 95%
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Official Symbol: LT
• Synonyms: Large T Antigen; LT; LT-AG; Simian virus 40 large T antigen