Recombinant Ovine Trophoblast Protein-1

• Cat.No.: TP-1-38O
• Product Overview: Recombinant Ovine Interferon-Tau produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 172 amino acids and having a molecular mass of 19914.7 Dalton. The IFN-Tau is purified by proprietary chromatographic techniques.

Specification

• Species: Ovine
• Source: E.coli
• Tag: Non
• Description: IFN-tau is also known as TP-1 (trophoblast protein-1) is a new class of type I IFN that is secreted by the trophoblast and is the signal for maternal recognition of pregnancy in sheep. IFN- tau has potent immunosuppressive and antiviral activities similar to other type I IFN but is less cytotoxic than IFN-alpha and IFN-beta. The current investigation concerns the effect of recombinant ovine IFN- tau (rOIFN- tau) on the modulation of MHC class I and II expression on cloned mouse cerebrovascular endothelial (CVE) cells. IFN-tau induced tyrosine phosphorylation of Stat1 and upregulated the expression of MHC class I on CVE. One proposed action by which type I IFN reduces the relapse rate in MS is via interference with IFN-γ-induced MHC class II expression. IFN- tau was shown to downregulate IFN-γ-induced MHC class II expression on CVE and, hence, may be of potential therapeutic value in downregulating inflammation in the central nervous system (CNS). IFN- tau did not upregulate the expression of MHC class II on CVE. IFN- tau also inhibited the replication of Theiler"s virus in CVE.
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Tyr-Leu-Ser-Arg.
• Biological Activity: The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 1 x 10⁷ IU/mg.
• Hysical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: Lyophilized from (1mg/ml) solution containing PBS pH-7.4.
• Solubility: It is recommended to reconstitute the lyophilized Interferon Tau in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.027 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IFN-Tau as a Reference Standard.
• Stability: Lyophilized Interferon-Tau although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-Tau should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: TP-1 trophoblast protein-1 [ Ovis aries ]
• Synonyms: Interferon-tau; TP-1; trophoblast protein-1 ; Trophoblastin; Antiluteolysin; Trophoblast antiluteolytic protein; OTP; IFNT1; IFNT4; Interferon tau-1; Interferon tau-4
• Gene ID: 100144750
• mRNA Refseq: NM_001123399
• Protein Refseq: NP_001116871
• UniProt ID: P56828