Recombinant Ovine Growth Hormone, Antagonist

• Cat.No.: GH-36O
• Product Overview: Recombinant Ovine Somatotropin Antagonist G119R produced inE.Coliis a single, non-glycosylated polypeptide chain containing 191 amino acids and having a molecular mass of 22 kDa. Recombinant Ovine Somatotropin Antagonist is purified by proprietary chromatographic techniques.

Specification

• Species: Ovine
• Source: E.coli
• Tag: Non
• Description: GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Phe-Pro-Ala.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045 mM NaHCO₃.
• Solubility: It is recommended to reconstitute the lyophilized GH G119R in 0.4% NaHCO₃or water adjusted to pH 9, not less than 100 µg/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.
• Specific Activity: GH G119R acts as antagonist using an in vitro bioassay in PDF-P1 3B9 cells stably transfected with rabbit GH receptors. It is capable of forming a 1:1 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD) and binds to this ECD with affinity similar the the wild type oGH.
• Protein Content: Protein quantitation was carried out by: UV spectroscopy at 280 nm using the absorbency value of 0.66 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
• Stability: Lyophilized GH G119R although stable at room temperature for at least two weeks, should be stored desiccated below -18°C. Upon reconstitution and filter sterilization GH can be stored at 4°C, pH 9 for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: GH growth hormone [ Ovis aries ]
• Synonyms: GH; growth hormone; GH1; somatotropin
• Gene ID: 443329
• mRNA Refseq: NM_001009315
• Protein Refseq: NP_001009315
• UniProt ID: P67930