Recombinant O'nyong-nyong virus E2 protein, His-tagged

• Cat.No.: E2-01O
• Product Overview: Recombinant O'nyong-nyong virus E2 protein (a.a. 338-691) with C-terminal 6xHis tag was expressed in E.coli.

Specification

• Species: O'nyong-nyong virus
• Source: E.coli
• Tag: His
• Protein Length: 338-691 a.a.
• Description: Spike glycoprotein E2: Plays a role in viral attachment to target host cell, by binding to the cell receptor. Synthesized as a p62 precursor which is processed by furin at the cell membrane just before virion budding, giving rise to E2-E1 heterodimer. The p62-E1 heterodimer is stable, whereas E2-E1 is unstable and dissociate at low pH. p62 is processed at the last step, presumably to avoid E1 fusion activation before its final export to cell surface. E2 C-terminus contains a transitory transmembrane that would be disrupted by palmitoylation, resulting in reorientation of the C-terminal tail from lumenal to cytoplasmic side. This step is critical since E2 C-terminus is involved in budding by interacting with capsid proteins. This release of E2 C-terminus in cytoplasm occurs lately in protein export, and precludes premature assembly of particles at the endoplasmic reticulum membrane.
• Purity: >95% , based on SDS PAGE
• Applications: WB standard, antibody ELISA, immunogen, etc.
• Notes: For research use only, not for use in diagnostic procedures.
• Stability: Stable for 6 months at -20 centigrade from the date of shipment. Nonhazardous.
• Storage Buffer: Each vial contains 100 µg of protein in PBS with 8M Urea.

Gene Information

• UniProt ID: O90369