Recombinant Myobacterium Tuberculosis Molecular Chaperone DnaK

• Cat.No.: DnaK-1257H
• Product Overview: Recombinant Mycobacterium Tuberculosis Dnak produced inE.Coliis a single, non-glycosylated polypeptide chain containing 625 amino acids and having a molecular mass of 66.7 kDa.

Specification

• Species: Mycobacterium Tuberculosis
• Source: E.coli
• Tag: Non
• Description: DnaK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The DnaK protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM sodium phosphate buffer pH 7.4, 130mM sodium chloride and 2.5mM KCl.
• Solubility: It is recommended to reconstitute the lyophilized DnaK in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Stability: Lyophilized DnaK although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DnaK should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Pathways: RNA degradation

Gene Information

• Gene Name: dnaK molecular chaperone DnaK [ Mycobacterium tuberculosis H37Rv ]
• Synonyms: Rv0350; molecular chaperone DnaK
• Gene ID: 885946
• Protein Refseq: NP_214864