Recombinant Mycobacterium tuberculosis kshB Protein (M1-E358)

• Cat.No.: kshB-1433M
• Product Overview: Recombinant Mycobacterium tuberculosis GG-KSHB(M1-E358 end) (TB enzyme) protein without tag was expressed in E. coli

Specification

• Species: Mycobacterium tuberculosis
• Source: E.coli
• Tag: Non
• ProteinLength: M1-E358
• Description: Involved in the degradation of cholesterol. Catalyzes the introduction of a 9a-hydroxyl moiety into 1,4-androstadiene-3,17-dione (ADD) to yield the 9alpha-hydroxy-1,4-androstadiene-3,17-dione (9OHADD) intermediate which spontaneously form 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione (HSA) via the meta-cleavage of ring B with concomitant aromatization of ring A. KSH is also able to use 4-androstene-3,17-dione (AD), 3-oxo-23,24-bisnorcholesta-4-en-22-oate (4-BNC), 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate (1,4-BNC), 3-oxo-23,24-bisnorcholesta-4-en-22-oyl-coenzyme A thioester (4-BNC-CoA) and 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-coenzyme A thioester (1,4-BNC-CoA) as substrates.
• Form: Liquid
• Endotoxin: < 0.01 EU/μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50mM Tris-HCl (pH 7.5), 200mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: kshB 3-ketosteroid-9-alpha-hydroxylase reductase subunit [ Mycobacterium tuberculosis H37Rv ]
• Official Symbol: kshB
• Synonyms: kshB; 3-ketosteroid-9-alpha-hydroxylase reductase subunit; hmp; 3-ketosteroid-9-alpha-hydroxylase reductase subunit
• Gene ID: 887315
• Protein Refseq: NP_218088
• UniProt ID: P9WJ93