Recombinant Mouse Zfp207 Protein, Myc/DDK-tagged

• Cat.No.: Zfp207-7080M
• Product Overview: Purified recombinant protein of Mouse zinc finger protein 207 (Zfp207), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Kinetochore- and microtubule-binding protein that plays a key role in spindle assembly. ZNF207/BuGZ is mainly composed of disordered low-complexity regions and undergoes phase transition or coacervation to form temperature-dependent liquid droplets. Coacervation promotes microtubule bundling and concentrates tubulin, promoting microtubule polymerization and assembly of spindle and spindle matrix by concentrating its building blocks. Also acts as a regulator of mitotic chromosome alignment by mediating the stability and kinetochore loading of BUB3. Mechanisms by which BUB3 is protected are unclear: according to a first report, ZNF207/BuGZ may act by blocking ubiquitination and proteasomal degradation of BUB3. According to another report, the stabilization is independent of the proteasome.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 49.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Zfp207 zinc finger protein 207 [ Mus musculus (house mouse) ]
• Official Symbol: Zfp207
• Synonyms: Zfp207; zinc finger protein 207; Ze; Zep; BuGZ; Znf207; 8430401D15Rik; BUB3-interacting and GLEBS motif-containing protein ZNF207; 49 kDa zinc finger protein
• Gene ID: 22680
• mRNA Refseq: NM_001130171
• Protein Refseq: NP_001123643
• UniProt ID: Q9JMD0