Recombinant Mouse Zer1 Protein, Myc/DDK-tagged

• Cat.No.: Zer1-7073M
• Product Overview: Purified recombinant protein of Mouse zyg-11 related, cell cycle regulator (Zer1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Serves as substrate adapter subunit in the E3 ubiquitin ligase complex ZYG11B-CUL2-Elongin BC. Acts redudantly with ZYG11B to target substrates bearing N-terminal glycine degrons for proteasomal degradation. Involved in the clearance of proteolytic fragments generated by caspase cleavage during apoptosis since N-terminal glycine degrons are strongly enriched at caspase cleavage sites. Also important in the quality control of protein N-myristoylation in which N-terminal glycine degrons are conditionally exposed after a failure of N-myristoylation.
• Molecular Mass: 89.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Zer1 zyg-11 related, cell cycle regulator [ Mus musculus (house mouse) ]
• Official Symbol: Zer1
• Synonyms: ZER1; zer-1 homolog (C. elegans); protein zer-1 homolog; zyg-11 homolog B-like protein; Zyg11bl; C230075L19Rik
• Gene ID: 227693
• mRNA Refseq: NM_178694
• Protein Refseq: NP_848809
• UniProt ID: Q80ZJ6