Recombinant Mouse Wrap53 Protein, Myc/DDK-tagged

• Cat.No.: Wrap53-7008M
• Product Overview: Purified recombinant protein of Mouse WD repeat containing, antisense to Trp53 (Wrap53), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: RNA chaperone that plays a key role in telomere maintenance and RNA localization to Cajal bodies. Specifically recognizes and binds the Cajal body box (CAB box) present in both small Cajal body RNAs (scaRNAs) and telomerase RNA template component (TERC). Essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex essential for the replication of chromosome termini that elongates telomeres in most eukaryotes. In the telomerase holoenzyme complex, required to stimulate the catalytic activity of the complex. Acts by specifically binding the CAB box of the TERC RNA and controlling the folding of the CR4/CR5 region of the TERC RNA, a critical step for telomerase activity. In addition, also controls telomerase holoenzyme complex localization to Cajal body. During S phase, required for delivery of TERC to telomeres during S phase and for telomerase activity. In addition to its role in telomere maintenance, also required for Cajal body formation, probably by mediating localization of scaRNAs to Cajal bodies. Also plays a role in DNA repair: phosphorylated by ATM in response to DNA damage and relocalizes to sites of DNA double-strand breaks to promote the repair of DNA double-strand breaks. Acts by recruiting the ubiquitin ligase RNF8 to DNA breaks and promote both homologous recombination (HR) and non-homologous end joining (NHEJ).
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 58.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Wrap53 WD repeat containing, antisense to Trp53 [ Mus musculus (house mouse) ]
• Official Symbol: Wrap53
• Synonyms: WRAP53; WD repeat containing, antisense to TP53; telomerase Cajal body protein 1; WD repeat domain 79; WD repeat-containing protein 79; WD40 repeat-containing protein encoded by RNA antisense to p53; Wdr79; BC021790; RP23-56I20.3; MGC28622
• Gene ID: 216853
• mRNA Refseq: NM_144824
• Protein Refseq: NP_659073
• UniProt ID: Q8VC51