Recombinant Mouse Usp44 Protein, Myc/DDK-tagged

• Cat.No.: Usp44-6880M
• Product Overview: Purified recombinant protein of mouse full-length ubiquitin specific peptidase 44 (Usp44), transcript variant 2, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 58 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Usp44 ubiquitin specific peptidase 44 [ Mus musculus (house mouse) ]
• Official Symbol: Usp44
• Synonyms: Usp44; ubiquitin specific peptidase 44; E430004F17Rik; ubiquitin carboxyl-terminal hydrolase 44; deubiquitinating enzyme 44; inactive deubiquitinating enzyme 44; inactive ubiquitin carboxyl-terminal hydrolase 44; inactive ubiquitin thioesterase 44; inactive ubiquitin-specific-processing protease 44; ubiquitin specific peptidase 49 pseudogene; ubiquitin thioesterase 44; EC 3.4.19.12
• Gene ID: 327799
• mRNA Refseq: NM_183199
• UniProt ID: Q8C2S0