Recombinant Mouse USAG1 Protein, Full Length

• Cat.No.: Sostdc1-01MFL
• Product Overview: Recombinant Full Length Mouse USAG1 Protein with an N-terminal Met was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Protein Length: Full Length
• Description: Predicted to enable BMP binding activity and BMP receptor activity. Acts upstream of or within several processes, including animal organ development; negative regulation of cell fate commitment; and negative regulation of signal transduction. Located in extracellular space. Is expressed in several structures, including alimentary system; genitourinary system; limb; nervous system; and sensory organ. Orthologous to human SOSTDC1 (sclerostin domain containing 1).
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Human USAG1 is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse LRP-6 that produces 50% of the optimal binding response is approximately 0.1-0.6 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 21 kDa
  SDS-PAGE: 21 kDa, reducing conditions
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• N-terminal Sequence Analysis: Met
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in HCl.
• Shipping: The product is shipped at ambient temperature.
• Reconstitution: Reconstitute at 250 μg/mL in 4 mM HCl.
• References: 1. Laurikkala, J. et al. (2003) Dev. Biol. 264:91.
  2. Yanagita, M. et al. (2004) Biochem. Biophys. Res. Commun. 316:490.
  3. Lintern, K.B. et al. (2009) J. Biol. Chem. 284:23159.

Gene Information

• Gene Name: Sostdc1 sclerostin domain containing 1 [ Mus musculus (house mouse) ]
• Official Symbol: Sostdc1
• Synonyms: Sostdc1; sclerostin domain containing 1; Wise; Sostl; USAG-1; ectodin; 0610006G05Rik; sclerostin domain-containing protein 1; cystine-knot containing secreted protein; ectodermal BMP inhibitor; sclerostin-like protein; uterine sensitization-associated gene 1 protein; uterine sensitization-associated protein 1
• Gene ID: 66042
• mRNA Refseq: NM_025312
• Protein Refseq: NP_079588
• UniProt ID: Q9CQN4