Recombinant Mouse Ufc1 Protein, Myc/DDK-tagged

• Cat.No.: Ufc1-6824M
• Product Overview: Purified recombinant protein of mouse full-length ubiquitin-fold modifier conjugating enzyme 1 (Ufc1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: E1-like enzyme which specifically catalyzes the second step in ufmylation. Accepts the ubiquitin-like modifier UFM1 from the E1 enzyme UBA5 and forms an intermediate with UFM1 via a thioester linkage. Ufmylation is involved in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 19.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ufc1 ubiquitin-fold modifier conjugating enzyme 1 [ Mus musculus (house mouse) ]
• Official Symbol: Ufc1
• Synonyms: UFC1; ubiquitin-fold modifier conjugating enzyme 1; ubiquitin-fold modifier-conjugating enzyme 1; Ufm1-conjugating enzyme 1; ESTM29; AA062310; 1110021H02Rik
• Gene ID: 66155
• mRNA Refseq: NM_025388
• Protein Refseq: NP_079664
• UniProt ID: Q9CR09