Recombinant Mouse Ubxn1 Protein, Myc/DDK-tagged

• Cat.No.: Ubxn1-6812M
• Product Overview: Purified recombinant protein of mouse full-length UBX domain protein 1 (Ubxn1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Ubiquitin-binding protein that plays a role in the modulation of innate immune response. Blocks both the RIG-I-like receptors (RLR) and NF-kappa-B pathways. Following viral infection, UBXN1 is induced and recruited to the RLR component MAVS. In turn, interferes with MAVS oligomerization, and disrupts the MAVS/TRAF3/TRAF6 signalosome. This function probably serves as a brake to prevent excessive RLR signaling. Interferes with the TNFalpha-triggered NF-kappa-B pathway by interacting with cellular inhibitors of apoptosis proteins (cIAPs) and thereby inhibiting their recruitment to TNFR1. Prevents also the activation of NF-kappa-B by associating with CUL1 and thus inhibiting NF-kappa-B inhibitor alpha/NFKBIA degradation that remains bound to NF-kappa-B. Interacts with the BRCA1-BARD1 heterodimer and regulates its activity. Specifically binds 'Lys-6'-linked polyubiquitin chains. Interaction with autoubiquitinated BRCA1 leads to the inhibition of the E3 ubiquitin-protein ligase activity of the BRCA1-BARD1 heterodimer. Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 34 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ubxn1 UBX domain protein 1 [ Mus musculus (house mouse) ]
• Official Symbol: Ubxn1
• Synonyms: UBXN1; UBX domain protein 1; UBX domain-containing protein 1; mY33K; protein 2B28; SAPK substrate protein 1; UBA/UBX 33.3 kDa protein; 2B28; T25529; D19Ertd721e; MGC6696; 4930455J02Rik
• Gene ID: 225896
• mRNA Refseq: NM_146093
• Protein Refseq: NP_666205
• UniProt ID: Q922Y1