Recombinant Mouse Ttc8 Protein, Myc/DDK-tagged

• Cat.No.: Ttc8-6715M
• Product Overview: Purified recombinant protein of mouse full-length tetratricopeptide repeat domain 8 (Ttc8), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 58.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ttc8 tetratricopeptide repeat domain 8 [ Mus musculus (house mouse) ]
• Official Symbol: Ttc8
• Synonyms: TTC8; tetratricopeptide repeat domain 8; tetratricopeptide repeat protein 8; TPR repeat protein 8; bardet-Biedl syndrome 8 protein homolog; BBS8; AV001447; 0610012F22Rik
• Gene ID: 76260
• mRNA Refseq: NM_198311
• Protein Refseq: NP_938053
• UniProt ID: Q8VD72