Recombinant Mouse Trp53bp1 Protein, Myc/DDK-tagged

• Cat.No.: Trp53bp1-6672M
• Product Overview: Purified recombinant protein of mouse full-length transformation related protein 53 binding protein 1 (cDNA clone MGC:30704 IMAGE:3979746), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis. Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1. In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs. Participates in the repair and the orientation of the broken DNA ends during CSR. In contrast, it is not required for classic NHEJ and V(D)J recombination. Promotes NHEJ of dysfunctional telomeres.
• Molecular Mass: 109.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Trp53bp1 transformation related protein 53 binding protein 1 [ Mus musculus (house mouse) ]
• Official Symbol: Trp53bp1
• Synonyms: Trp53bp1; transformation related protein 53 binding protein 1; 53BP; p53B; 53BP1; m53BP1; p53BP1; Tp53bp1; TP53-binding protein 1; murine p53-binding protein; p53-binding protein 1; tumor suppressor p53-binding protein 1
• Gene ID: 27223
• mRNA Refseq: NM_013735
• Protein Refseq: NP_038763
• UniProt ID: P70399