Recombinant Mouse Trmu Protein, Myc/DDK-tagged

• Cat.No.: Trmu-6669M
• Product Overview: Purified recombinant protein of mouse full-length tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (Trmu), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.
• Molecular Mass: 47.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Trmu tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase [ Mus musculus (house mouse) ]
• Official Symbol: Trmu
• Synonyms: Trmu; tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase; Trmt; Trmt1; AI314320; 1110005N20Rik; 1600025P05Rik; mitochondrial tRNA-specific 2-thiouridylase 1; tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase 1; EC 2.8.1.14
• Gene ID: 72026
• mRNA Refseq: NM_028063
• Protein Refseq: NP_082339
• UniProt ID: Q9DAT5