Recombinant Mouse Trim13 Protein, Myc/DDK-tagged

• Cat.No.: Trim13-6644M
• Product Overview: Purified recombinant protein of mouse full-length tripartite motif-containing 13 (Trim13), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: Endoplasmic reticulum (ER) membrane anchored E3 ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER-associated degradation (ERAD). This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins. Enhances ionizing radiation-induced p53/TP53 stability and apoptosis via ubiquitinating MDM2 and AKT1 and decreasing AKT1 kinase activity through MDM2 and AKT1 proteasomal degradation. Regulates ER stress-induced autophagy, and may act as a tumor suppressor. Plays also a role in innate immune response by stimulating NF-kappa-B activity in the TLR2 signaling pathway. Ubiquitinates TRAF6 via the 'Lys-29'-linked polyubiquitination chain resulting in NF-kappa-B activation. Participates as well in T-cell receptor-mediated NF-kappa-B activation. In the presence of TNF, modulates the IKK complex by regulating IKBKG/NEMO ubiquitination leading to the repression of NF-kappa-B.
• Molecular Mass: 47.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Trim13 tripartite motif-containing 13 [ Mus musculus (house mouse) ]
• Official Symbol: Trim13
• Synonyms: TRIM13; tripartite motif-containing 13; E3 ubiquitin-protein ligase TRIM13; ret finger protein 2; tripartite motif protein 13; putative tumor suppressor RFP2; tripartite motif-containing protein 13; CAR; LEU5; Rfp2; RNF77; 3110001L12Rik
• Gene ID: 66597
• mRNA Refseq: NM_023233
• Protein Refseq: NP_075722
• UniProt ID: Q9CYB0