Recombinant Mouse Top2a protein, His & T7-tagged

• Cat.No.: Top2a-7702M
• Product Overview: Recombinant Mouse Top2a aa. (Ser749~Val987 (Accession # Q01320)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His&T7
• Protein Length: Ser749~Val987
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 31.0kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.9.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Top2a topoisomerase (DNA) II alpha [ Mus musculus (house mouse) ]
• Official Symbol: Top2a
• Synonyms: Top-2; DNA Topoisomerase II alpha; DNA topoisomerase II, alpha isozyme; DNA topoisomerase 2-alpha
• Gene ID: 21973
• mRNA Refseq: NM_011623.2
• Protein Refseq: NP_035753.2
• UniProt ID: Q01320