Recombinant Mouse Tnfsf5, FLAG-tagged

• Cat.No.: Tnfsf5-2077M
• Product Overview: Recombinant Mouse Tnfsf5 is produced inHEK 293 Cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG-tag. MW=26kDa.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Flag
• Protein Length: 115-260 a.a.
• Description: CD154, also called CD40 ligand or CD40L, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In general, CD40L plays the role of a costimulatory molecule and induces activation in APC in association with T cell receptor stimulation by MHC molecules on the APC. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper IgM syndrome.
• Purity: ≥90% (SDS-PAGE).
• Concentration: 0.1mg/ml after reconstitution.
• Formulation: Lyophilized. Contains PBS.
• Endotoxin Content: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
• Reconstitution: Reconstitute each tube with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
• Specificity: Binds to human and mouse CD40.
• Biological Activity: Stimulates the proliferation of mouse B cells and dendritic cells. The activity of rmsCD40L increases 1"000-fold (stimulation in the ng/ml range) in the presence of a cross-linking enhancer.
• Storage: -20°C.

Gene Information

• Gene Name: Cd40lg CD40 ligand [ Mus musculus ]
• Synonyms: Cd40lg; CD40 ligand; IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; Tnfsf5; OTTMUSP00000019349; tumor necrosis factor (ligand) superfamily, member 5
• Gene ID: 21947
• mRNA Refseq: NM_011616
• Protein Refseq: NP_035746
• UniProt ID: P27548
• Chromosome Location: X A5; X 18.0 cM
• Pathway: Allograft rejection; Asthma; Autoimmune thyroid disease; Cell adhesion molecules (CAMs); Cytokine-cytokine receptor interaction; Primary immunodeficiency; Systemic lupus erythematosus; T cell receptor signaling pathway
• Function: cytokine activity; tumor necrosis factor receptor binding