Recombinant Mouse Tnfsf13, FLAG-tagged

• Cat.No.: Tnfsf13-401M
• Product Overview: The extracellular domain of mouse APRIL (aa 98-232) is fused at the N-terminus to mouse ACRP30headless (aa 18-110) and a FLAG-tag.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Flag
• Description: APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth, is involved in monocyte/macrophage-mediated immunological processes and functions as an important survival factor for plasmablasts and bone marrow plasma cells. MegaAPRIL is a high activity construct in which two trimeric APRIL ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively stimulates proliferation B cell. A basic amino acid sequence (QKQKKQ) close to the NH2 terminus of APRIL is required for binding to negatively charged sulfated glycosaminoglycan side chains of proteoglycans. Proteoglycans mediate binding of APRIL to tumor cells as well as primary lymphoid cells.
• Form: Lyophilized. Contains PBS + 0.5% Trehalose.
• Bio-activity: Stimulates B cell proliferation.
• Purity: ≥95% (SDS-PAGE)
• Stability: Stable for at least 6 months after receipt when stored at -20°C.
• Storage: Short Term Storage: +4°C; Long Term Storage: -20°C. After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions.
• Concentration: 0.1mg/ml after reconstitution.

Gene Information

• Gene Name: Tnfsf13 tumor necrosis factor (ligand) superfamily, member 13 [ Mus musculus ]
• Official Symbol: Tnfsf13
• Synonyms: TNFSF13; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand superfamily member 13; a proliferation-inducing ligand; April; Tall2; Trdl1; 2310026N09Rik; MGC106105
• Gene ID: 69583
• mRNA Refseq: NM_001159505
• Protein Refseq: NP_001152977
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Intestinal immune network for IgA production, organism-specific biosystem; Intestinal immune network for IgA production, conserved biosystem; Rheumatoid arthritis, organism-specific biosystem; Rheumatoid arthritis, conserved biosystem
• Function: cytokine activity; tumor necrosis factor receptor binding