Recombinant Mouse Tnfsf12, FLAG-tagged

• Cat.No.: Tnfsf12-196M
• Product Overview: Recombinant Mouse Tnfsf12 isproduced inHEK 293 cells.The extracellular domain of mouse TWEAK (aa 94-249) is fused at the N-terminus to tenascin-C (TNC) (chicken) (aa 110-139) and a FLAG-tag.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Flag
• Protein Length: 94-249;110-139 a.a.
• Description: Some TNF ligands (such as TWEAK (mouse) or FasL (mouse)) produced as soluble forms do not assemble into stable trimers and show low receptor binding. Addition of the tenascin-C oligomerization domain (TNC) (from chicken) at the N-terminus of recombinant mouse soluble TWEAK stabilizes the TNF homology domain (THD) of rmsTWEAK into a trimer and increases binding to its respective TNF receptor.
• Purity: ≥95% (SDS-PAGE).
• Endotoxin Content: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
• Concentration: 0.1mg/ml after reconstitution.
• Formulation: Lyophilized. Contains PBS.
• Reconstitution: Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
• Stability: Stable for at least 6 months after receipt when stored at -20°C.
• Storage: Long term storage: -20°C, short term storage: +4°C. After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: Tnfsf12 tumor necrosis factor (ligand) superfamily, member 12 [ Mus musculus ]
• Synonyms:
  Tnfsf12; tumor necrosis factor (ligand) superfamily, member 12; DR3L; APO3L; DR3LG; Tweak; tumor necrosis factor ligand superfamily member 12; OTTMUSP00000006216; TNF-related weak inducer of apoptosis
• Gene ID: 21944
• mRNA Refseq: NM_011614
• Protein Refseq: NP_035744
• UniProt ID: O54907
• Chromosome Location: 11
• Pathway: Cytokine-cytokine receptor interaction
• Function: cytokine activity; tumor necrosis factor receptor binding