Recombinant Mouse Tlr4 protein, His & S-tagged

• Cat.No.: Tlr4-7619M
• Product Overview: Recombinant Mouse Tlr4 aa. (Asn26~Glu269 (Accession # Q9QUK6)) fused with N-terminal His & S tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His&S
• Protein Length: Asn26~Glu269
• Description: This gene belongs to the evolutionarily-conserved Toll-like receptor family, whose members are type-1 transmembrane proteins that are involved in innate immunity. Toll-like receptors are characterized by an extracellular leucine-rich repeat domain that functions in ligand recognition and an intracellular toll/interleukin-1 receptor-like domain that is crucial for signal transduction. The receptor encoded by this gene mediates the innate immune response to bacterial lipopolysaccharide, a major component of the outer membrane of Gram-negative bacteria, through synthesis of pro-inflammatory cytokines and chemokines. In addition, this protein can recognize other pathogens from Gram-negative and Gram-positive bacteria as well as viral components. Mice deficient in this gene display a number of immune response-related phenotypes including hyporesponsiveness to bacterial lipopolysaccharide and increased levels of respiratory syncytial virus compared to controls.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 33.3kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.7.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Tlr4 toll-like receptor 4 [ Mus musculus (house mouse) ]
• Official Symbol: Tlr4
• Synonyms: Lps; Ly87; Ran/M1; Rasl2-8; Toll like receptor 4; lipopolysaccharide response; toll-like receptor 4
• Gene ID: 21898
• mRNA Refseq: NM_021297.3
• Protein Refseq: NP_067272.1
• UniProt ID: Q9QUK6