Recombinant Mouse Th protein, His-tagged

• Cat.No.: Th-25M
• Product Overview: Recombinant mouse Th, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: His
• Description: Th, also known as tyrosine 3-monooxygenase, is a rate-limiting enzyme in catecholamine synthesis. It uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to DOPA. It regulates dopamine (DA) neurotransmission at the biosynthesis and reuptake steps. It plays an important role in the physiology of adrenergic neurons. It effects overexpression in lymphocytes on the differentiation and function of T helper cells.
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 57.0kDa (507aa)
  50-70kDa (SDS-PAGE under reducing conditions)
• AA Sequence: ADPMPTPSAS SPQPKGFRRA VSEQDTKQAE AVTSPRFIGR RQSLIEDARK EREAAAAAAA AAVASAEPGN PLEAVVFEER DGNAVLNLLFSLRGTKPSSL SRALKVFETF EAKIHHLETR PAQRPLAGSP HLEYFVRFEV PSGDLAALLS SVRRVSDDVR SAREDKVPWF PRKVSELDKCHHLVTKFDPD LDLDHPGFSD QAYRQRRKLI AEIAFQYKQG EPIPHVEYTK EEIATWKEVY ATLKGLYATH ACREHLEAFQ LLERYCGYREDSIPQLEDVS HFLKERTGFQ LRPVAGLLSA RDFLASLAFR VFQCTQYIRH ASSPMHSPEP DCCHELLGHV PMLADRTFAQ FSQDIGLASL
  GASDEEIEKL STVYWFTVEF GLCKQNGELK AYGAGLLSSY GELLHSLSEE PEVRAFDPDT AAVQPYQDQT YQPVYFVSES FSDAKDKLRNYASRIQRPFS VKFDPYTLAI DVLDSPHTIR RSLEGVQDEL HTLTQALSAI SHHHHHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: > 90% by SDS - PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20centigrade or -70centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: Th tyrosine hydroxylase [ Mus musculus ]
• Official Symbol: Th
• Synonyms: TH; tyrosine hydroxylase; tyrosine 3-monooxygenase; tyrosine 3-hydroxylase
• Gene ID: 21823
• mRNA Refseq: NM_009377
• Protein Refseq: NP_033403
• Pathway: Amine-derived hormones, organism-specific biosystem; Amphetamine addiction, organism-specific biosystem; Amphetamine addiction, conserved biosystem; Biogenic Amine Synthesis, organism-specific biosystem; Catecholamine biosynthesis, organism-specific biosystem; Catecholamine biosynthesis, tyrosine => dopamine =>
• Function: amino acid binding; dopamine binding; ferric iron binding; ferrous iron binding; iron ion binding; metal ion binding; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen; oxygen binding; protein domain specific binding; tetrahydrobiopterin binding; tyrosine 3-monooxygenase activity; tyrosine 3-monooxygenase activity