Recombinant Mouse Tgm3 Protein, Myc/DDK-tagged

• Cat.No.: Tgm3-6398M
• Product Overview: Purified recombinant protein of mouse full-length transglutaminase 3, E polypeptide (Tgm3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold. In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 77.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Tgm3 transglutaminase 3, E polypeptide [ Mus musculus (house mouse) ]
• Official Symbol: Tgm3
• Synonyms: TGM3; transglutaminase 3, E polypeptide; protein-glutamine gamma-glutamyltransferase E; TGE; TG E; TG(E); TGase E; TGase-3; E polypeptide; transglutaminase E; transglutaminase-3; protein-glutamine-gamma-glutamyltransferase; AI893889; MGC150256; MGC150257
• Gene ID: 21818
• mRNA Refseq: NM_009374
• Protein Refseq: NP_033400
• UniProt ID: Q08189