Recombinant Mouse Ssbp1 Protein, Myc/DDK-tagged

• Cat.No.: Ssbp1-6140M
• Product Overview: Purified recombinant protein of mouse full-length single-stranded DNA binding protein 1 (Ssbp1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA). In vitro, required to maintain the copy number of mitochondrial DNA (mtDNA) and plays crucial roles during mtDNA replication that stimulate activity of the replisome components POLG and TWNK at the replication fork. Promotes the activity of the gamma complex polymerase POLG, largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate POLG activity. In addition it is able to promote the 5'-3' unwinding activity of the mtDNA helicase TWNK. May also function in mtDNA repair.
• Molecular Mass: 17.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ssbp1 single-stranded DNA binding protein 1 [ Mus musculus (house mouse) ]
• Official Symbol: Ssbp1
• Synonyms: SSBP1; single-stranded DNA binding protein 1; single-stranded DNA-binding protein, mitochondrial; mt-SSB; MtSSB; mtDBP; 2810480P10Rik; G630031O20Rik
• Gene ID: 381760
• mRNA Refseq: NM_212468
• Protein Refseq: NP_997633
• UniProt ID: Q9CYR0