Recombinant Mouse Spire2 Protein, Myc/DDK-tagged

• Cat.No.: Spire2-6101M
• Product Overview: Purified recombinant protein of mouse full-length spire type actin nucleation factor 2 (Spire2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament. Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport. Required for asymmetric spindle positioning and asymmetric cell division during oocyte meiosis. Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis. Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage.
• Molecular Mass: 80.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Spire2 spire type actin nucleation factor 2 [ Mus musculus (house mouse) ]
• Official Symbol: Spire2
• Synonyms: SPIRE2; spire homolog 2 (Drosophila); protein spire homolog 2; Spir2; Spir-2; BC026502; KIAA1832; MGC31610
• Gene ID: 234857
• mRNA Refseq: NM_172287
• Protein Refseq: NP_758491
• UniProt ID: Q8K1S6