Recombinant Mouse Sphk2 Protein, His-tagged

• Cat.No.: Sphk2-2093M
• Product Overview: Recombinant Mouse Sphk2 Protein (Gly18-Leu219) with N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. The gene product also enhances apoptosis in different cell types and suppresses cellular proliferation. In mast cells, the encoded protein is necessary for influx of calcium, protein kinase C activation, and cytokine production and degranulation. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 26.2 kDa
  Accurate Molecular Mass: 28 kDa
• Protein length: Gly18-Leu219
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: Sphk2 sphingosine kinase 2 [ Mus musculus (house mouse) ]
• Official Symbol: Sphk2
• Synonyms: SPHK2; sphingosine kinase 2; SK 2; SPK 2; Spk2, Sk2; C76851; MGC102297
• Gene ID: 56632
• mRNA Refseq: NM_001172561
• Protein Refseq: NP_001166032
• UniProt ID: Q9JIA7