Recombinant Mouse Spata18 Protein, Myc/DDK-tagged

• Cat.No.: Spata18-6076M
• Product Overview: Purified recombinant protein of mouse full-length spermatogenesis associated 18 (Spata18), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Key regulator of mitochondrial quality that mediates the repairing or degradation of unhealthy mitochondria in response to mitochondrial damage. Mediator of mitochondrial protein catabolic process (also named MALM) by mediating the degradation of damaged proteins inside mitochondria by promoting the accumulation in the mitochondrial matrix of hydrolases that are characteristic of the lysosomal lumen. Also involved in mitochondrion degradation of damaged mitochondria by promoting the formation of vacuole-like structures (named MIV), which engulf and degrade unhealthy mitochondria by accumulating lysosomes. May have a role in spermatogenesis, especially in cell differentiation from late elongate spermatids to mature spermatozoa. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 60.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Spata18 spermatogenesis associated 18 [ Mus musculus (house mouse) ]
• Official Symbol: Spata18
• Synonyms: SPATA18; spermatogenesis associated 18; mitochondria-eating protein; spermatogenesis associated 18 homolog; spermatogenesis-associated protein 18; 1700067I02Rik
• Gene ID: 73472
• mRNA Refseq: NM_178387
• Protein Refseq: NP_848474
• UniProt ID: Q0P557