Recombinant Mouse Smpd3 Protein, His-tagged

• Cat.No.: Smpd3-2086M
• Product Overview: Recombinant Mouse Smpd3 Protein (Asp415-Glu652) with N-His tag was expressed in E. coli.

Specification

• Description: Enables identical protein binding activity; neutral sphingomyelin phosphodiesterase activity; and phospholipid binding activity. Involved in sphingomyelin metabolic process. Acts upstream of or within several processes, including animal organ development; enzyme linked receptor protein signaling pathway; and sphingolipid metabolic process. Located in Golgi cis cisterna and plasma membrane. Is expressed in several structures, including alimentary system; limb; nervous system; sensory organ; and skeleton. Used to study hypopituitarism; osteogenesis imperfecta; osteogenesis imperfecta type 2; and osteogenesis imperfecta type 3. Orthologous to human SMPD3 (sphingomyelin phosphodiesterase 3).
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 30.1 kDa
  Accurate Molecular Mass: 33 kDa
• Protein length: Asp415-Glu652
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Smpd3 sphingomyelin phosphodiesterase 3, neutral [ Mus musculus (house mouse) ]
• Official Symbol: Smpd3
• Synonyms: SMPD3; sphingomyelin phosphodiesterase 3, neutral; sphingomyelin phosphodiesterase 3; nSMase-2; neutral sphingomyelinase 2; neutral sphingomyelinase II; fro; nSMase2; AI427456; AW537966; 4631433G07Rik
• Gene ID: 58994
• mRNA Refseq: NM_021491
• Protein Refseq: NP_067466
• UniProt ID: Q9JJY3