Recombinant Mouse Smarce1 Protein, Myc/DDK-tagged

• Cat.No.: Smarce1-5963M
• Product Overview: Purified recombinant protein of mouse full-length SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 (cDNA clone, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells. Required for the coactivation of estrogen responsive promoters by SWI/SNF complexes and the SRC/p160 family of histone acetyltransferases (HATs).
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 46.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Smarce1 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 [ Mus musculus (house mouse) ]
• Official Symbol: Smarce1
• Synonyms: Smarce1; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1; BAF5; Baf27; 2810417B20Rik; 5830412H02Rik; 9030408N19Rik; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1; BAF57; BRG1-associated factor 57; SWI/SNF-related matrix-associated actin-dependent regulator chromatin subfamily E member 1
• Gene ID: 57376
• mRNA Refseq: NM_020618
• Protein Refseq: NP_065643
• UniProt ID: O54941