Recombinant Mouse Sirt3 protein, His-tagged

• Cat.No.: Sirt3-1375M
• Product Overview: Recombinant Mouse Sirt3 aa. (Gly5~Arg257 fused with N-terminal His tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 29/16kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Gly5~Arg257
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 6.4.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Sirt3 sirtuin 3 [ Mus musculus (house mouse) ]
• Official Symbol: Sirt3
• Synonyms: Sirt3; sirtuin 3; Sir2l3; AI848213; 2310003L23Rik; NAD-dependent protein deacetylase sirtuin-3; NAD-dependent deacetylase sirtuin-3; SIR2-like protein 3; SIRT3L mitochondrial; mSIR2L3; mitochondrial protein lysine deacetylase; regulatory protein SIR2 homolog 3; silent mating type information regulation 2, (S.cerevisiae, homolog)-like 3
• Gene ID: 64384
• mRNA Refseq: NM_001127351.1
• Protein Refseq: NP_001120823.1
• UniProt ID: Q8R104