Active Recombinant Human SIRT3, His-tagged

• Cat.No.: SIRT3-346H
• Product Overview: Recombinant Human SIRT3(Ser101-Lys399), fused with an N-terminal Met and 6-His tag, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 101-399 a.a.
• Description: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.
• Predicted N Terminal: Met
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl, Brij-35 and Glycerol.
• Bio-activity: Measured by its ability to remove the acetyl group from a fluorogenic peptide substrate Ac-RGK(Ac)-AMC in a coupled assay.
• Molecular Mass: Predicted Molecular Mass: 34 kDaSDS-PAGE: 30-33 kDa, reducing conditions
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 °C as supplied.3 months, -20 to -70 °C under sterile conditions after opening.

Gene Information

• Gene Name: SIRT3 sirtuin 3 [ Homo sapiens (human) ]
• Official Symbol: SIRT3
• Synonyms: SIRT3; sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae); SIR2L3; NAD-dependent deacetylase sirtuin-3, mitochondrial; EC 3.5.1.-; SIR2-like protein 3; hSIRT3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase 2; silent mating type information regulation 2, S.cerevisiae, homolog 3; sir2-like 3; sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3; sirtuin 3; sirtuin type 3
• Gene ID: 23410
• mRNA Refseq: NM_001017524
• Protein Refseq: NP_001017524
• MIM: 604481
• UniProt ID: Q9NTG7
• Chromosome Location: 11p15.5
• Pathway: Energy Metabolism; Mitochondrial biogenesis; Organelle biogenesis and maintenance
• Function: NOT NAD+ ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity (H3-K14 specific); protein binding