Recombinant Mouse SFRP1 protein(Met1-Lys314), His-tagged

• Cat.No.: SFRP1-790M
• Product Overview: Recombinant Mouse SFRP1 (AAC53145.1) (Met1-Lys314) was expressed in CHO with a polyhistidine tag at the C-terminus.

Specification

• Source: CHO
• Species: Mouse
• Tag: His
• Protein length: Met1-Lys314
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.4-2 μg/mL in the presence of 300 ng/mL of Recombinant Mouse Wnt‑3a.
• Molecular Mass: The recombinant mouse SFRP1 consists of 283 amino acids and predicts a molecular mass of 32.6 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Sfrp1 secreted frizzled-related protein 1 [ Mus musculus ]
• Official Symbol: SFRP1
• Synonyms: SFRP1; secreted frizzled-related protein 1; secreted frizzled-related sequence protein 1; sFRP-1; AW011917; AW107218; AW742929; 2210415K03Rik
• Gene ID: 20377
• mRNA Refseq: NM_013834
• Protein Refseq: NP_038862