Active Recombinant Human SEMA3A protein, Fc-tagged
Cat.No. : | SEMA3A-14H |
Product Overview : | Recombinant Human SEMA3A protein(Lys 26 - Val 771), fused with Fc tag, was expressed in HEK293. |
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Species : | Human |
Source : | HEK293 |
Tag : | Fc |
Protein Length : | Lys 26 - Val 771 |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose. |
Bio-activity : | Immobilized Human Neuropilin-1, His Tag at 5 μg/mL (100 μL/well) can bind Human Semaphorin 3A, Fc Tag with a linear range of 2-78 ng/mL. Immobilized Biotinylated Human Neuropilin-1, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human Semaphorin 3A, Fc Tag with a linear range of 0.6-20 ng/mL. |
Molecular Mass : | Molecular CharacterizationThis protein carries a human IgG1 Fc tag at the N-terminus.The protein has a calculated MW of 111.8 kDa. The protein migrates as 120-135 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS. |
Storage : | For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name | SEMA3A sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A [ Homo sapiens ] |
Official Symbol | SEMA3A |
Synonyms | SEMA3A; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A; SEMAD; semaphorin-3A; coll 1; Hsema I; sema III; SEMA1; SemD; collapsin 1; semaphorin D; semaphorin L; semaphorin III; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III; MGC133243; |
Gene ID | 10371 |
mRNA Refseq | NM_006080 |
Protein Refseq | NP_006071 |
MIM | 603961 |
UniProt ID | Q14563 |
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SEMA3A-1901H | Recombinant Human SEMA3A Protein, His-tagged | +Inquiry |
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◆ Cell & Tissue Lysates | ||
SEMA3A-1770MCL | Recombinant Mouse SEMA3A cell lysate | +Inquiry |
Not For Human Consumption!
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