Active Recombinant Human SEMA3A protein, Fc-tagged

• Cat.No.: SEMA3A-14H
• Product Overview: Recombinant Human SEMA3A protein(Lys 26 - Val 771), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Human Neuropilin-1, His Tag at 5 μg/mL (100 μL/well) can bind Human Semaphorin 3A, Fc Tag with a linear range of 2-78 ng/mL. Immobilized Biotinylated Human Neuropilin-1, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human Semaphorin 3A, Fc Tag with a linear range of 0.6-20 ng/mL.
• Molecular Mass: Molecular CharacterizationThis protein carries a human IgG1 Fc tag at the N-terminus.The protein has a calculated MW of 111.8 kDa. The protein migrates as 120-135 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Lys 26 - Val 771
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SEMA3A sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A [ Homo sapiens ]
• Official Symbol: SEMA3A
• Synonyms: SEMA3A; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A; SEMAD; semaphorin-3A; coll 1; Hsema I; sema III; SEMA1; SemD; collapsin 1; semaphorin D; semaphorin L; semaphorin III; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III; MGC133243
• Gene ID: 10371
• mRNA Refseq: NM_006080
• Protein Refseq: NP_006071
• MIM: 603961
• UniProt ID: Q14563