Recombinant Mouse Sav1 Protein, Myc/DDK-tagged

• Cat.No.: Sav1-5697M
• Product Overview: Purified recombinant protein of mouse full-length salvador family WW domain containing 1 (Sav1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: Regulator of STK3/MST2 and STK4/MST1 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. SAV1 is required for STK3/MST2 and STK4/MST1 activation and promotes cell-cycle exit and terminal differentiation in developing epithelial tissues. Plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with STK3/MST2, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation.
• Molecular Mass: 44.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Sav1 salvador family WW domain containing 1 [ Mus musculus (house mouse) ]
• Official Symbol: Sav1
• Synonyms: SAV1; salvador homolog 1 (Drosophila); protein salvador homolog 1; 45 kDa WW domain protein; WW domain-containing protein 3; WW domain-containing protein 4; Sav; Salv; WW45; Wwp3; Wwp4; 1700040G09Rik
• Gene ID: 64010
• mRNA Refseq: NM_022028
• Protein Refseq: NP_071311
• UniProt ID: Q8VEB2