Recombinant Mouse Rragd Protein, Myc/DDK-tagged

• Cat.No.: Rragd-5622M
• Product Overview: Purified recombinant protein of mouse full-length Ras-related GTP binding D (Rragd), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RRAGA or RRAGB and cycles between an inactive GTP-bound and an active GDP-bound form. In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids.
• Molecular Mass: 45.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rragd Ras-related GTP binding D [ Mus musculus (house mouse) ]
• Official Symbol: Rragd
• Synonyms: RRAGD; Ras-related GTP binding D; ras-related GTP-binding protein D; ragD; rag D; AI467523; D4Ertd174e; 5730543C08Rik; C030003H22Rik
• Gene ID: 52187
• mRNA Refseq: NM_027491
• Protein Refseq: NP_081767
• UniProt ID: Q7TT45