Recombinant Mouse Rpe65 Protein, Myc/DDK-tagged

• Cat.No.: Rpe65-5581M
• Product Overview: Purified recombinant protein of mouse full-length retinal pigment epithelium 65 (Rpe65), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore. Essential for the production of 11-cis retinal for both rod and cone photoreceptors. Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT.
• Molecular Mass: 61.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rpe65 retinal pigment epithelium 65 [ Mus musculus (house mouse) ]
• Official Symbol: Rpe65
• Synonyms: RPE65; retinal pigment epithelium 65; retinoid isomerohydrolase; retinol isomerase; modifier of retinal degeneration 1; retinal pigment epithelium, 65 kDa; all-trans-retinyl-palmitate hydrolase; retinal pigment epithelium-specific 65 kDa protein; LCA2; RP20; rd12; 65kDa; Mord1; A930029L06Rik
• Gene ID: 19892
• mRNA Refseq: NM_029987
• Protein Refseq: NP_084263
• UniProt ID: Q91ZQ5