Recombinant Mouse Rpa1 Protein, Myc/DDK-tagged

• Cat.No.: Rpa1-5578M
• Product Overview: Purified recombinant protein of mouse full-length replication protein A1 (Rpa1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.
• Molecular Mass: 69 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rpa1 replication protein A1 [ Mus musculus (house mouse) ]
• Official Symbol: Rpa1
• Synonyms: RPA1; replication protein A1; replication protein A 70 kDa DNA-binding subunit; RP-A p70; RF-A protein 1; replication factor A protein 1; Rpa; RF-A; RP-A; 70kDa; AA589576; AW557552; 5031405K23Rik
• Gene ID: 68275
• mRNA Refseq: NM_026653
• Protein Refseq: NP_080929
• UniProt ID: Q8VEE4