Recombinant Mouse Rnf41 Protein, Myc/DDK-tagged

• Cat.No.: Rnf41-5559M
• Product Overview: Purified recombinant protein of mouse full-length ring finger protein 41 (cDNA clone MGC:30902 IMAGE:4014525), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Acts as E3 ubiquitin-protein ligase and regulates the degradation of target proteins. Polyubiquitinates MYD88. Negatively regulates MYD88-dependent production of proinflammatory cytokines. Can promote TRIF-dependent production of type I interferon and inhibits infection with vesicular stomatitis virus. Promotes also activation of TBK1 and IRF3. Involved in the ubiquitination of erythropoietin (EPO) and interleukin-3 (IL-3) receptors. Thus, through maintaining basal levels of cytokine receptors, RNF41 is involved in the control of hematopoietic progenitor cell differentiation into myeloerythroid lineages. Contributes to the maintenance of steady-state ERBB3 levels by mediating its growth factor-independent degradation. Involved in the degradation of the inhibitor of apoptosis BIRC6 and thus is an important regulator of cell death by promoting apoptosis. Acts also as a PRKN modifier that accelerates its degradation, resulting in a reduction of PRKN activity, influencing the balance of intracellular redox state. The RNF41-PRKN pathway regulates autophagosome-lysosome fusion during late mitophagy. Mitophagy is a selective form of autophagy necessary for mitochondrial quality control.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 22.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rnf41 ring finger protein 41 [ Mus musculus (house mouse) ]
• Official Symbol: Rnf41
• Synonyms: Rnf41; ring finger protein 41; FL; Nr; FLRF; Nrdp1; D10Ertd722; D10Ertd722e; 2210404G21Rik; 4930511A05Rik; 4933415P08Rik; E3 ubiquitin-protein ligase NRDP1; RING-type E3 ubiquitin transferase NRDP1; EC 2.3.2.27
• Gene ID: 67588
• mRNA Refseq: NM_026259
• Protein Refseq: NP_080535
• UniProt ID: Q8BH75