Recombinant Mouse Rnf4 protein, His & GST-tagged

• Cat.No.: Rnf4-1654M
• Product Overview: Recombinant Mouse Rnf4 aa. (Met1~Ile194 (Accession # Q9QZS2)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: This gene encodes a small nuclear RING finger protein that mediates ubiquitylation of polysumoylated proteins. Deficiency of the encoded protein in mice leads to embryonic lethality and global DNA hypermethylation. A similar protein in humans is required for arsenic-induced degradation of promyelocytic leukemia protein in acute promyelocytic leukemia. Alternative splicing of this gene results in multiple transcript variants. A pseudogene for this gene has been identified on chromosome 10.
• Source: E. coli
• Species: Mouse
• Tag: His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 51.9kDa
• Protein length: Met1~Ile194
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.
• Isoelectric Point: 7.6

Gene Information

• Gene Name: Rnf4 ring finger protein 4 [ Mus musculus (house mouse) ]
• Official Symbol: Rnf4
• Synonyms: Rnf4; ring finger protein 4; SNURF; Gtrgeo8; AU018689; E3 ubiquitin-protein ligase RNF4; E3 ubiquitin ligase RNF4; RING-type E3 ubiquitin transferase RNF4
• Gene ID: 19822
• mRNA Refseq: NM_001304269.1
• Protein Refseq: NP_001291198.1
• UniProt ID: Q9QZS2