Recombinant Mouse Rnf34 Protein, Myc/DDK-tagged

• Cat.No.: Rnf34-5557M
• Product Overview: Purified recombinant protein of mouse full-length ring finger protein 34 (Rnf34), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis. May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells. Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation.
• Molecular Mass: 42 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rnf34 ring finger protein 34 [ Mus musculus (house mouse) ]
• Official Symbol: Rnf34
• Synonyms: RNF34; ring finger protein 34; E3 ubiquitin-protein ligase RNF34; phafin 1; phafin-1; RING finger protein RIFF; RIFF; C88279; AW061037; AW536122; BC004042; MGC7670
• Gene ID: 80751
• mRNA Refseq: NM_030564
• Protein Refseq: NP_085041
• UniProt ID: Q99KR6