Recombinant Mouse Rnf185 Protein, Myc/DDK-tagged

• Cat.No.: Rnf185-5553M
• Product Overview: Purified recombinant protein of mouse full-length ring finger protein 185 (cDNA clone MGC:19394 IMAGE:3153870), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1. Acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation. Protects cells from ER stress-induced apoptosis. Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway. Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2.
• Molecular Mass: 20.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rnf185 ring finger protein 185 [ Mus musculus (house mouse) ]
• Official Symbol: Rnf185
• Synonyms: Rnf185; ring finger protein 185; E3 ubiquitin-protein ligase RNF185; RING-type E3 ubiquitin transferase RNF185; EC 2.3.2.27
• Gene ID: 193670
• mRNA Refseq: NM_145355
• Protein Refseq: NP_663330
• UniProt ID: Q91YT2