Recombinant Mouse Rnf11 Protein, Myc/DDK-tagged

• Cat.No.: Rnf11-5541M
• Product Overview: Purified recombinant protein of mouse full-length ring finger protein 11 (Rnf11), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of TNFAIP3 to RIPK1 after TNF stimulation. TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Recruits STAMBP to the E3 ubiquitin-ligase SMURF2 for ubiquitination, leading to its degradation by the 26S proteasome.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 17.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rnf11 ring finger protein 11 [ Mus musculus (house mouse) ]
• Official Symbol: Rnf11
• Synonyms: RNF11; ring finger protein 11; RING finger protein 11; sid 1669; NEDD4 WW domain-binding protein 2
• Gene ID: 29864
• mRNA Refseq: NM_013876
• Protein Refseq: NP_038904
• UniProt ID: Q9QYK7