Recombinant Mouse Rab8a Protein, Myc/DDK-tagged

• Cat.No.: Rab8a-5332M
• Product Overview: Purified recombinant protein of mouse full-length RAB8A, member RAS oncogene family (Rab8a), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Myc&DDK
• Description: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. May be involved in ciliogenesis. Together with MICALL2, may also regulate adherens junction assembly. May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis. Involved in autophagy.
• Molecular Mass: 24.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rab8a RAB8A, member RAS oncogene family [ Mus musculus (house mouse) ]
• Official Symbol: Rab8a
• Synonyms: RAB8A; RAB8A, member RAS oncogene family; ras-related protein Rab-8A; oncogene c-mel; cell line NK14 derived transforming oncogene; Mel; AA409338
• Gene ID: 17274
• mRNA Refseq: NM_023126
• Protein Refseq: NP_075615
• UniProt ID: P55258