Recombinant Mouse Rab3a Protein, Myc/DDK-tagged

• Cat.No.: Rab3a-5317M
• Product Overview: Purified recombinant protein of mouse full-length RAB3A, member RAS oncogene family (Rab3a), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane. Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane. Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed. Stimulates insulin secretion through interaction with RIMS2 isoform RIMS2 and RPH3AL effectors in pancreatic beta cells. Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9. Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1. Plays a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 25 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rab3a RAB3A, member RAS oncogene family [ Mus musculus (house mouse) ]
• Official Symbol: Rab3a
• Synonyms: RAB3A; RAB3A, member RAS oncogene family; ras-related protein Rab-3A
• Gene ID: 19339
• mRNA Refseq: NM_009001
• Protein Refseq: NP_033027
• UniProt ID: P63011