Recombinant Mouse Pygm protein, His-tagged

• Cat.No.: Pygm-7822M
• Product Overview: Recombinant Mouse Pygm aa. (Arg11~Gly187) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Arg11~Gly187
• Description: This gene encodes a glycolysis enzyme found in muscle. Highly similar enzymes encoded by different genes are found in liver and brain. The encoded protein is involved in regulating the breakdown of glycogen to glucose-1-phosphate, which is necessary for ATP generation.
• Form: Freeze-dried powder
• Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >98%
• Characteristic: The isoelectric point is 6.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification;
  Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Pygm muscle glycogen phosphorylase [ Mus musculus (house mouse) ]
• Official Symbol: Pygm
• Synonyms: Pygm; muscle glycogen phosphorylase; PG; AI115133; glycogen phosphorylase, muscle form; myophosphorylase
• Gene ID: 19309
• mRNA Refseq: NM_011224.2
• Protein Refseq: NP_035354.1
• UniProt ID: Q9WUB3