Recombinant Mouse PYCARD protein, His-tagged

• Cat.No.: PYCARD-8966M
• Product Overview: Recombinant Mouse PYCARD protein(1-193 aa), fused with N-terminal His tag, was expressed in E. coli.

Specification

• Species: Mouse
• Source: E. coli
• Tag: His
• Protein Length: 1-193 aa
• Form: The purified protein was lyophilized from sterile phosphate-buffered saline (PBS: 58 mM Na₂HPO₄, 17 mM NaH₂PO₄, 68 mM NaCl, pH 7.4). Prior to lyophilization, 5% (w/v) trehalose and 5% (w/v) mannitol were incorporated as cryoprotective excipients. The protein was eluted with a buffer containing 300 mM imidazole.
• AASequence: MGRARDAILDALENLSGDELKKFKMKLLTVQLREGYGRIPRGALLQMDAIDLTDKLVSYYLESYGLELTMTVLRDMGLQELAEQLQTTKEESGAVAAAASVPAQSTARTGHFVDQHRQALIARVTEVDGVLDALHGSVLTEGQYQAVRAETTSQDKMRKLFSFVPSWNLTCKDSLLQALKEIHPYLVMDLEQS
• Purity: 85%, by SDS-PAGE.
• Storage: Store at 2-8°C for 1-2 weeks. Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Pycard PYD and CARD domain containing [ Mus musculus ]
• Official Symbol: PYCARD
• Synonyms: PYCARD; PYD and CARD domain containing; apoptosis-associated speck-like protein containing a CARD; PYD and CARD domain-containing protein; Asc; TNS1; masc; CARD5; TMS-1; 9130417A21Rik
• mRNA Refseq: NM_023258
• Protein Refseq: NP_075747
• Gene ID: 66824