Recombinant Mouse Ptges2 protein, His-tagged

• Cat.No.: Ptges2-5624M
• Product Overview: Recombinant Mouse Ptges2 aa. (Tyr144~Asp384) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Tyr144~Asp384
• Description: The protein encoded by this gene is a Golgi membrane-associated prostaglandin E synthase candidate, which is capable of catalyzing the conversion of prostaglandin H2 to prostaglandin E2 in vitro. However, a study using mice deficient of this gene suggests that this enzyme does not contribute to prostaglandin E2 biosynthesis in vivo. This protein is synthesized as a Golgi membrane-bound protein, but its N-terminal hydrophobic region is cleaved off during protein maturation to produce the predominant soluble truncated form that still retains the enzyme activity. This soluble protein also has been shown to activate the transcription regulated by a gamma-interferon-activated transcription element (GATE), possibly via an interaction with CAAAT/enhancer-binding protein-beta.
• Form: Freeze-dried powder
• Molecular Mass: 30kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >98%
• Characteristic: The isoelectric point is 6.4.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Ptges2 prostaglandin E synthase 2 [ Mus musculus (house mouse) ]
• Official Symbol: Ptges2
• Synonyms: Ptges2; prostaglandin E synthase 2; Gbf1; Pges2; C79137; Mpges2; 0610038H10Rik; prostaglandin E synthase 2; GATE-binding factor 1; mPGES-2; microsomal prostaglandin E synthase 2
• Gene ID: 96979
• mRNA Refseq: NM_133783.2
• Protein Refseq: NP_598544.2
• UniProt ID: Q8BWM0